Mitochondrial NADH-Ubiquinone Oxidoreductase (Complex I)

Diphenyleneiodonium inhibits reduction of iron-sulfur clusters in the mitochondrial NADH-ubiquinone oxidoreductase (Complex I).

Diphenyleneiodonium (DPI) inhibits the mitochondrial NADH-ubiquinone oxidoreductase (Complex I) on the substrate side of the Fe-S clusters. In the inhibited NADH-supplemented state all of the Fe-S clusters are oxidized, whereas the reduced minus oxidized difference spectrum of the protein-bound FMN can be visualized. It is characterized by troughs at 370 and 450 nm and a small increase of absor...

Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency.

Human complex I (NADH:ubiquinone oxidoreductase; EC 1.6.5.3) is the first and largest multi-protein assembly of the mitochondrial oxidative phosphorylation (OXPHOS) system; the final biochemical cascade of events leading to the production of ATP. The complex consists of 46 subunits, 7 encoded by the mitochondrial DNA and the remainder by the nuclear genome. In recent years, numerous gene mutati...

3D Reconstruction of a Subcomplex of NADH-ubiquinone-oxidoreductase (Complex I) from Yarrowia lipolytica.

Complex I is the first and largest enzyme in the respiratory chain located in the inner mitochondrial membrane and in the membrane of many bacteria [1]. In the process of oxidizing NADH, Complex I translocates 4 protons across the membrane. This is followed by additional proton translocation by complexes III and IV. The resulting membrane potential powers ATP production by the F-ATPase. The min...

Electron and Proton Transfer in NADH: Ubiquinone Oxidoreductase (Complex I) from Escherichia coli

Origin and Evolution of the Sodium -Pumping NADH: Ubiquinone Oxidoreductase

The sodium -pumping NADH: ubiquinone oxidoreductase (Na+-NQR) is the main ion pump and the primary entry site for electrons into the respiratory chain of many different types of pathogenic bacteria. This enzymatic complex creates a transmembrane gradient of sodium that is used by the cell to sustain ionic homeostasis, nutrient transport, ATP synthesis, flagellum rotation and other essential pro...